- in service
- United States Marine Corps (USMC)
- F-35A - Conventional Take-Off and Landing (CTOL) variant (for USAF)
- F-35B - Short Take-Off and Vertical Landing (STOVL) variant (for USMC)
- F-35C - Catapult Assisted Take-Off But Arrested Recovery (CATOBAR) variant (for USN)
- Marine Fighter Attack Squadron 121 (VMFA-121) "Green Knights" - United States Marine Corps (USMC) aircraft squadron operating the F-35B Lightning II. The squadron is based at Marine Corps Air Station Iwakuni, Japan
- Marine Fighter Attack Squadron 211 (VMFA-211) "Wake Island Avengers" - United States Marine Corps (USMC) aircraft squadron operating the F-35B Lightning II. The squadron is based at Marine Corps Air Station Yuma, Arizona
- Marine Fighter Attack Training Squadron 501 (VMFAT-501) "Warloads" - training squadron in the United States Marine Corps, consisting of 28 F-35B Lightning II. The squadron is based at Marine Corps Air Station Beaufort, South Carolina. The squadron has assumed the lineage of VMFA-451 “Blue Devils” and saw action during World War II and Operation Desert Storm. They were decommissioned on January 31, 1997.
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